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"Morphological maturation of the mouse brain: An in vivo MRI and histology investigation"

Luam Hammelrath, Siniša Škokić, Artem Khmelinskii, Andreas Hess, Noortje van der Knaap, Marius Staring, Boudewijn P.F. Lelieveldt, Dirk Wiedermann and Mathias Hoehn

Abstract

With the wide access to studies of selected gene expressions in transgenic animals, mice have become the dominant species as cerebral disease models.Many of these studies are performed on animals of not more than eight weeks, declared as adult animals. Based on the earlier reports that full brain maturation requires at least three months in rats, there is a clear need to discern the corresponding minimal animal age to provide an "adult brain" in mice in order to avoid modulation of disease progression/therapy studies by ongoing developmental changes. For this purpose, we have studied anatomical brain alterations of mice during their first six months of age. Using T2-weighted and diffusion-weighted MRI, structural and volume changes of the brain were identified and compared with histological analysis of myelination. Mouse brain volume was found to be almost stable already at three weeks, but cortex thickness kept decreasing continuously with maximal changes during the first three months. Myelination is still increasing between three and six months, although most dramatic changes are over by three months. While our results emphasize that mice should be at least three months old when adult animals are needed for brain studies, preferred choice of one particular metric for future investigation goals will result in somewhat varying age windows of stabilization.

 

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Copyright © 2016 by the authors. Published version © 2016 by Elsevier Science. Personal use of this material is permitted. However, permission to reprint or republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works, must be obtained from the copyright holder.

 

BibTeX entry

@article{Hammelrath:2016,
author = "{Luam Hammelrath and Siniša Škokić and Artem Khmelinskii and Andreas Hess and Noortje van der Knaap and Marius Staring and Boudewijn P.F. Lelieveldt and Dirk Wiedermann and Mathias Hoehn}",
title = "{Morphological maturation of the mouse brain: An in vivo MRI and histology investigation}",
journal = "{NeuroImage}",
volume = "{125}",
number = "{15}",
pages = "{144 - 152}",
month = "{January}",
year = "{2016}",
}

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